On. (A, B) Cells were pretreated with BAPTA/AM or TG SEO BOOKs
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On. (A, B) Cells were pretreated with BAPTA/AM or TG for 1 h and then incubated with 50 g/ml LTA for 24 h. Conditioned media were collected and analyzed by gelatin zymography. The cell lysates were assayed by western blot using an anti-GAPDH antibody as a control. (C-E) For Ca2+ mobilization, confluent cultures of RBA-1 cells on glass coverslips were loaded with Fura-2/AM and fluorescent measureme